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Label Free Quantitative Proteomics Approach Unravels the Pleotropy of Buffalo Leukemia Inhibitory Factor (BuLIF) in COS-1 cells

Sudarshan Kumar*, Syed Azmal Ali, and Ashok Kumar Mohanty

Leukemia Inhibitory Factor (LIF) may be a pleotropic molecule which performs diverse functions during a context dependent manner. Bovine LIF (BuLIF) is an important media component in in-vitro somatic cell culture and also considered essential within the early stages of pregnancy. However, the exact molecular mechanism behind the diverse actions of this molecule is unknown except the stat3 mediated canonical pathways in stem cell pluripotency. One of the "cells that are self-replicating, are derived from human embryos or human fetal tissue, and are known to become cells and tissues of the three primary germ layers. Although human pluripotent stem cells could also be derived from embryos or fetal tissue, such stem cells aren't themselves embryos." (From the National Institutes of Health Guidelines for Research Using Human Pluripotent Stem Cells.)

"Self-replicating" means the cell can divide and to make cells indistinguishable from it. The three primary germ layers (called the ectoderm, mesoderm, and endoderm) are the first layers of cells within the embryo from which all tissues and organs develop. Human pluripotent stem cells also are referred to as human embryonic stem cells.