False results perceptions of different urine parameters delaying one day for analysis

Rehman Zafar, Imran Zafar, Muhammad Fahid, Muhammad Zubair, Wajeeha Waseem, Khadija Shahid, Samar Akhtar, Hammad Ahmad and Hashaam Akhtar

Background: Numerous laboratory facilities are certainly challenging in scrutinizing great quantity of urinary samples that reach laboratory in the late evening and are frozen till next day. On the other hand, various clinics and small medical centers might face difficulties in guaranteeing same day test sample deliveries and analysis. As the stability of authentication of urinary test results from post ponding the samples creates much differences in results, mechanized dipstick investigation Supertron is anonymous, we repeated the tests from the overnight refrigerated urine samples and compared the characterizing accuracy. Objectives: The purpose of this Clinical research is to identify the risk of having the false positive and negative results when urine analysis is delayed for almost one day due to different circumstances. Method: Urine analyzer (Supertron) was used in this project and urine samples were tested through dipstick immediately after receiving the samples and were reanalyzed after one day refrigeration (4°C). Both results were compared using SPSS. Results: Urine was analyzed for leukocyte esterase, hemoglobin, proteins, nitrites, ketone bodies and glucose values. Highly accurate values for leukocyte esterase were observed but the reactivity decreased to 25% of original after keeping for 24 hours. Hemoglobin accountability was observed to be high but similarly lowered with passage of 24 hours. Variation in results was observed for proteins quantifications and it raised. Analysis for glucose, ketones and nitrites also displayed variations in readings. Conclusion: Comparing variation in urinalysis results of instantly handled samples with the 24 hours delayed ones displays a lot of variation in the final readings, which raises a lot of question in the authentication of these procedures and lab protocols. Our current study elucidates that variation occurs in the final readings if the sample is kept for 24 hours and false negative or false positive results about Leukocyte concentration, nitrates and proteins could be detected but no such difference was observed in case of glucose and ketones.